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目的了解铜绿假单胞菌1型整合子携带情况及其与消毒剂抗性之间的关系。方法采用PCR技术对临床分离26株铜绿假单胞菌进行Ⅰ型整合子检测,用肉汤稀释法测定了上述菌株对消毒剂抗力。结果临床分离26株铜绿假单胞菌检出携带1型整合子16株,占61.5%。所有临床分离铜绿假单胞菌株对含碘消毒剂和苯扎氯铵两类消毒剂在常用使用浓度下均未产生抗性现象;但有38.5%的菌株对含氯消毒剂的M IC和MBC值增加。对含氯消毒剂M IC和MBC值增加的菌株中,有90%携带Ⅰ型整合子阳性。结论铜绿假单胞菌Ⅰ型整合子阳性携带率较高,其多数菌株对含氯消毒剂抗性增加。
Objective To understand the relationship between Pseudomonas aeruginosa type 1 integron carrier and disinfectant resistance. Methods PCR-based technique was used to detect type I integrants of 26 Pseudomonas aeruginosa isolates. The antibacterial activity of these strains against disinfectants was determined by broth dilution method. Results Twenty-six isolates of Pseudomonas aeruginosa were detected with 16 strains of type 1 integrons, accounting for 61.5%. All clinical isolates of Pseudomonas aeruginosa did not show any resistance to iodine disinfectant and benzalkonium chloride disinfectants at commonly used concentrations; however, 38.5% Value increased. Ninety percent of the strains that increased the value of M IC and MBC for chlorine-containing disinfectants were positive for type I integron. Conclusion The Pseudomonas aeruginosa type Ⅰ integron possesses higher positive rate of carriage, and most of the strains have increased resistance to chlorine-containing disinfectants.