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目的 探讨亚低温和 (或 )非氨酯对缺氧缺血 (hypoxia ischemia ,HI)脑损伤的保护作用。方法 将HI模型鼠 (右颈总动脉结扎后给予 8%氧气 2h)随机分为亚低温组 (脑温降低 4 5℃ ) :包括亚低温 2 4h组 (n =2 6 )、亚低温 72h组 (n =48)、非氨酯组 (n =48)、亚低温 2 4h与非氨酯联合治疗组(n =5 1)和安慰剂组 (n =48)。对照组 (n =48)为假手术动物。监测动物的肛温和脑温。以血浆S 10 0蛋白 (S 10 0 )和肌酸激酶脑型同功酶 (CK BB)水平、病理检查脑损伤分数 (BIS)、脑海马神经元死亡率 (HDNP)和水迷宫实验结果判断疗效。结果 脑温低于肛温 0 3～ 0 5℃ (r =0 99,P <0 0 1) ,亚低温 2 4h组、亚低温 72h组、联合组动物肛温降低了 4～ 5℃ ,非氨酯组与安慰剂组肛温维持在 36～37℃之间。安慰剂组血浆S 10 0、CK BB水平于HI后 2 4～ 48h达峰值 (分别为 1 2 1～ 1 2 4μg/L和 5 2 9～ 5 4 2IU/L ) ,为对照组的 2～ 3倍 ,各干预组的S 10 0和CK BB水平明显低于安慰剂组 (P <0 0 1)。HI后 72h、14d、6 0d亚低温 72h组BIS和HDNP降低程度为 5 8%、85 %、87% ,联合组为 5 6 %、77%、76 % ,亚低温 2 4h组为 35 %、5 8%、47% ,非氨酯组为 35 %、6 6 %、6 7%。空间记忆功能依次排列为对照组 >联合组 >亚低温 72h?
Objective To investigate the protective effects of mild hypothermia and / or non-urethane on hypoxic-ischemic (HI) brain damage. Methods HI rats were randomly divided into mild hypothermia group (brain temperature decreased 45 ℃), including mild hypothermia 24 hours group (n = 26), mild hypothermia 72 hours group (n = 48), non-urethane group (n = 48), mild hypothermia 24 hours and non-urethane combination group (n = 51) and placebo group (n = 48). The control group (n = 48) was a sham-operated animal. Animal rectal temperature and brain temperature monitoring. The levels of plasma S 10 0 and CK BB, BIS, hippocampal neuron death rate (HDNP) and water maze were determined Efficacy. Results The brain temperature was lower than the rectal temperature of 0 3 ~ 0 5 ℃ (r = 0 99, P 0 01), mild hypothermia 24h group, mild hypothermia 72h group. The rectal temperature in the combined group decreased by 4 ~ 5 ℃ Rectal urethral group and placebo group maintained at between 36 ~ 37 ℃. In the placebo group, plasma S 10 0 and CK BB levels peaked at 24-48 hrs after HI (112-1144μg / L and 529-542IU / L, respectively) 3 times. The levels of S 10 0 and CK BB in each intervention group were significantly lower than those in the placebo group (P 0 01). In HI 72 h, 14 d and 60 d, the reduction of BIS and HDNP was 58%, 85%, 87% in the 72 h group, 56%, 77%, 76% in the combined group and 35% 5 8%, 47%, non-urethane group 35%, 6 6%, 67%. Spatial memory function arranged in order of control group> combination group> mild hypothermia 72h?