论文部分内容阅读
采用大鼠整体灌流模型,同时测定灌流大鼠后肢血管床灌流压和全身平均动脉压,通过动态观察,比较多种α_1-肾上腺素受体(α_1-AR)亚型选择性拮抗剂对两者影响的异同,初步探讨α_1-AR亚型在大鼠整体血压调节中的作用。结果表明:α_1-AR选择性拮抗剂(prazosin组13.5±3.6vs 15.1±4.3,n=11)和α_1-AR亚型选择性拮抗剂(5-mithyl-urapidil组2.4±0.9vs 3.7±2.3,n=12;RS-17053组3.2±1.6vs 4.4±3.3,n=12)均对正常大鼠苯肾上腺素引起后肢血管床升压反应曲线和平均动脉压升压反应曲线的右移程度(dose ratio,Dr)无明显影响,α_1-AR亚型选择性拮抗剂(BMY 7378组1.9±0.9vs 2.2±0.8,n=8)对正常大鼠两者苯肾上腺素加压反应也无差别;自发性高血压大鼠(RS-17053组3.4±0.6vs 4.3±0.9,n=5;BMY 7378组1.7±0.5vs 1.7±0.5,n=8)的反应同正常大鼠相似。提示介导苯肾上腺素引起麻醉大鼠全身动脉加压效应的α_1-AR与引起大鼠后肢血管床收缩的α_1-AR可能是同一种亚型,即α_1-AR。
Rat global perfusion model was used to measure the perfusion pressure and systemic mean arterial pressure of the hind limbs of perfusion rats simultaneously. By comparing the effects of selective antagonists of α_1-adrenoceptor (α_1-AR) Effect of the similarities and differences, a preliminary study of α_1-AR subtype in the regulation of global blood pressure in rats. The results showed that α1-AR selective antagonist (prazosin group 13.5 ± 3.6 vs 15.1 ± 4.3, n = 11) and α1-AR subtype selective antagonist (2.4 ± 0.9 vs 3.7 ± 2.3, (n = 12; RS-17053 group 3.2 ± 1.6 vs 4.4 ± 3.3, n = 12), all of which showed that the phenylephrine-induced vasopressor response curve and mean arterial pressure- ratio, Dr), α_1-AR subtype selective antagonist (BMY 7378 group 1.9 ± 0.9 vs 2.2 ± 0.8, n = 8) had no difference in phenylephrine pressure response in normal rats; spontaneous Hypertensive rats (RS-17053 group 3.4 ± 0.6 vs 4.3 ± 0.9, n = 5; BMY 7378 group 1.7 ± 0.5 vs 1.7 ± 0.5, n = 8) were similar to those in normal rats. It is suggested that α_1-AR, which mediates phenylephrine-induced systemic arterial pressurization, may be the same subtype, α_1-AR, and α_1-AR, which causes contraction of vasculature in rat hindlimb.