Based on the adsorption of copper ions on single-walled carbon nanotubes(SWNTs) in electrolyte, Cu/SWNTs nanocomposite film was initially prepared on indium-doped tin oxide(ITO) substrate by one-step electrodeposition. This method may provide a versatile and facile pathway to fabricate other SWNTs-supported metal composite films. Electrochemical experiments revealed that the obtained Cu/SWNTs/ITO electrode offered an excellent electrocatalytic activity towards the oxidation of glucose and could be applied to the construction of non-enzymatic glucose biosensor. The linear range of the sensor was 1.0×10-6 to 6.0×10-4 mol/L and the response time was within 2 s. Particularly, its sensitivity reached as high as 1434.67 μA?L?mmol-1?cm-2, which was superior to any other non-enzymatic glucose biosensor based on copper-carbon nanotubes electrode reported previously. Based on the adsorption of copper ions on single-walled carbon nanotubes (SWNTs) in electrolyte, Cu / SWNTs nanocomposite film was initially prepared on indium-doped tin oxide (ITO) substrate by one-step electrodeposition. This method may provide a versatile and facile pathway to fabricate other SWNTs-supported metal composite films. Electrochemical experiments revealed that the obtained Cu / SWNTs / ITO electrode offered an excellent electrocatalytic activity towards the oxidation of glucose and could be applied to the construction of non-enzymatic glucose biosensor. The linear range of the sensor was 1.0 × 10 -6 to 6.0 × 10 -4 mol / L and the response time was within 2 s. Particularly, its sensitivity reached as high as 1434.67 μA • L • mmol -1 cm -2, which was superior to any other non-enzymatic glucose biosensor based on copper-carbon nanotubes electrode reported previously.