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目的:观察增液布津汤对干燥综合征(Sjgren’s syndrome,SS)模型鼠的治疗作用。方法:BALB/c小鼠60只,随机分为正常对照组、模型组、增液布津汤低、中、高剂量组和阳性药环戊硫酮组。除正常组外,其余5组均利用免疫诱导法(注射白百破疫苗+完全弗氏佐剂+大鼠颌下腺抗原)建立干燥综合征小鼠模型,于造模后当天正常对照组及模型组用生理盐水20 mL.kg-1ig,中药低、中、高剂量组用增液布津汤(相当于生药6.8,13.6,27.3 g.kg-1)ig,环戊硫酮组用环戊硫酮(1.25 g.kg-1)ig。首次免疫后第3,7天,用乳化抗原对模型组和用药组加强免疫,剂量同首次,多点背部sc。以后每隔14 d用乳化抗原加强免疫1次,剂量同首次,共加强免疫2次,多点背部sc,正常对照组不作任何处理。观察增液布津汤对模型小鼠体质量、颌下腺与胸腺、脾指数及唾液分泌量、颌下腺组织形态学变化的影响。结果:免疫小鼠颌下腺出现类似SS的病理改变,存在大量淋巴细胞浸润,提示免疫造模成功。模型组小鼠的体质量、唾液流量分别为(18.17±1.33)g,(7.51±1.25)mg,颌下腺指数、胸腺指数、脾指数分别为(3.02±0.98),(1.76±0.62),(6.93±2.03)mg.g-1,与正常对照组相比有显著性差异(P<0.01)。增液布津汤高剂量组小鼠体质量、唾液流量分别为(23.79±1.48)g,(11.05±1.93)mg,颌下腺指数、胸腺指数、脾指数分别为(4.24±0.84),(0.77±0.70),(4.58±1.39)mg.g-1,与模型组相比有显著性差异(P<0.01),余治疗组增加程度较增液布津汤高剂量组相比增加不明显。结论:增液布津汤可能通过抑制SS模型小鼠颌下腺萎缩,淋巴细胞浸润的病理进程,保护其唾液分泌功能,从而发挥对SS模型小鼠的治疗作用。
Objective: To observe the therapeutic effect of Zeng Bu Bu Jin Tang on Sjögren’s syndrome (SS) model rats. Methods: Sixty BALB / c mice were randomly divided into normal control group, model group, Zengbu Bu Jin Tang low, medium and high dose groups and positive drug cyclopentanthione group. In addition to the normal group, the other five groups were used to establish the mouse model of Sjogren’s syndrome by immune induction (injection of baibujifei vaccine + complete Freund’s adjuvant + rat submandibular gland antigen). On the day after modeling, the normal control group and model group With saline 20 mL.kg-1ig, traditional Chinese medicine low, medium and high dose group with liquid Bujin Tang (equivalent to crude drug 6.8,13.6,27.3 g.kg-1) ig, cyclopentanone group with cyclopentanthione (1.25 g.kg-1) ig. On the 3rd and 7th days after the first immunization, the model group and the medication group were boosted with the emulsified antigen at the same dose for the first time and with more back sc. After every 14 days with emulsified antigen to enhance immunity 1, the same dose for the first time, a total of 2 times to strengthen immunity, more back sc, the normal control group without any treatment. To observe the effect of Zeng Bu Bu Jin Tang on body weight, submandibular gland and thymus, spleen index and salivary secretion, histomorphology of submandibular gland in model mice. Results: SS-like pathological changes occurred in the submandibular gland of immunized mice, and there was a large number of infiltrating lymphocytes, suggesting that immune modeling was successful. The body mass and saliva flow in the model group were (18.17 ± 1.33) g and (7.51 ± 1.25) mg respectively, the submandibular gland index, thymus index and spleen index were (3.02 ± 0.98) and (1.76 ± 0.62) and ± 2.03) mg.g-1, which was significantly different from the normal control group (P <0.01). The body mass and saliva flow in the high dose Zeng Bu Bu Jin Tang group were (23.79 ± 1.48) g and (11.05 ± 1.93) mg respectively, the submandibular gland index, thymus index and spleen index were 4.24 ± 0.84 and 0.77 ± 0.70 ) And (4.58 ± 1.39) mg.g-1, respectively. Compared with the model group, there was a significant difference (P <0.01). Conclusion: Zeng Bu Bu Jin Tang may inhibit SS model mice by submandibular gland atrophy, lymphocyte infiltration of the pathological process to protect their salivary secretion function, and thus play a role in SS model mice.