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目的 :研究姜黄素对脓毒症小鼠急性肺损伤(acute Lung injury,ALI)的保护作用,探讨其作用机制。方法:动物随机分为手术组、假手术组、姜黄素组、卡托普利组和正常对照组,盲肠结扎穿孔术(cecal ligation and puncture,CLP)建立动物模型,利用光镜观察肺部组织形态学变化,称重法计算湿/干比(W/D),ELISA法检测血清中细胞因子TNF-α、IL-1β、IL-6水平,RIA法测定肺组织和血中AngⅡ的量。结果:与手术组比较,卡托普利(ACE拮抗剂)和姜黄素均可以使ALI/ARDS小鼠的氧合指数(259.5±24.2、268.8±21.5 vs 194.3±23.9)和W/D(5.35±0.25、5.13±0.59 vs 6.08±0.64)得到改善,都能不同程度地抑制体内AngⅡ的生成(肺:1.58±0.16、1.65±0.21 vs 2.38±0.41;血:178.04±17.87、153.74±10.24 vs 213.38±25.44),降低炎症因子水平,减轻肺组织病理学损伤。结论:姜黄素对脓毒症ALI具有保护作用,至少通过抑制炎症和抑制RAS两个重要机制发挥肺保护作用。
Objective: To study the protective effect of curcumin on acute lung injury (ALI) in septic mice and its mechanism. Methods: The animals were randomly divided into operation group, sham operation group, curcumin group, captopril group and normal control group, cecal ligation and puncture (CLP) to establish animal model, the use of light microscopy lung tissue Morphological changes were measured. The W / D ratio was calculated by weighing method. The levels of TNF-α, IL-1β and IL-6 in serum were detected by ELISA. The amount of AngⅡ in lung tissue and blood was determined by RIA. RESULTS: Captopril (ACE) antagonist and curcumin both improved oxygenation index (259.5 ± 24.2, 268.8 ± 21.5 vs 194.3 ± 23.9) and W / D (5.35 ± 0.25,5.13 ± 0.59 vs 6.08 ± 0.64), all of which could inhibit Ang Ⅱ production to a certain extent (lung: 1.58 ± 0.16,1.65 ± 0.21 vs 2.38 ± 0.41; blood: 178.04 ± 17.87,153.74 ± 10.24 vs 213.38 ± 25.44), reduce the level of inflammatory cytokines, reduce the pathological lung injury. CONCLUSION: Curcumin has a protective effect on ALI of sepsis and exerts lung protection through at least two important mechanisms of inhibiting inflammation and inhibiting RAS.