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天麻注射液是由天麻经提取制成的溶液,具有镇痉祛风、降压之功效。天麻素是其有效成分,但质量标准未对其含量进行测定~([1])。目前利用高效液相色谱法测定天麻素的含量报道较多~([2,3]),但用于天麻注射液的测定尚未见报道,我们利用高效液相色谱对天麻注射液中天麻素的含量进行了测定,结果表明,方法简便、快速,重现性、回收率均较满意。1仪器与试药1.1仪器:Waters高效液相色谱仪(包括510泵、490多功能紫外检测器)、HS色谱数据工作站(杭州英谱科技开发有限公司)。
Tianma injection is made by the solution made of Gastrodia elata, with antispasmodic Qufeng, antihypertensive effect. Gastrodin is an active ingredient, but the quality standard did not determine its content ~ ([1]). At present, the determination of gastrodin by high performance liquid chromatography more (~ 2,3), but for the determination of gastrodia injection has not been reported, we use high performance liquid chromatography gastrodin injection of gastrodin Content was measured, the results show that the method is simple, fast, reproducible, the recovery rate was satisfactory. 1 instruments and reagents 1.1 Instruments: Waters high performance liquid chromatography (including 510 pumps, 490 multi-function UV detector), HS chromatography data workstation (Hangzhou British spectrum Technology Development Co., Ltd.).