Adsorption resin AAS was used for the separation and purification of milupeinan (M-13) from its mother liquor. The good adsorption property of AAS adsorption resin was found for milupeinan (M-13) compared with the Diaion CHP 20p. When acetone and water (2:1 in volume) were used as desorption reagents, it was found that 3 times of bed volume of the desorption reagents would make the AAS resin which was saturately adsorpted M-13 desorption completely. Also a macroporous anion exchange resin (Poly-4-vinylpyridine type) D-280 was used for the decolourization from M-13 solution. Good results was given when the sv=1~2. The good adsorption property of AAS adsorption resin was found for milupeinan (M-13) compared with the Diaion CHP 20p. When acetone and water (M-13) was used for the separation and purification of milupein (2: 1 in volume) were used as desorption reagents, it was found that 3 times of bed volume of the desorption reagents would make the AAS resin which was saturately adsorbed M-13 desorption completely. Also a macroporous anion exchange resin (Poly- 4-vinylpyridine type D-280 was used for the decolourization from M-13 solution. Good results was given when the sv = 1-2.