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于1999年10月至2001年3月海湾扇贝(Argopectenirridians)大量死亡期间,对不同生长阶段的海湾扇贝进行了组织学及超微结构观察,在贝消化腺上皮细胞内发现嗜碱性的类衣原体包涵体,该包涵体为圆形及不规则形状,大小约9μm×7μm,HE染色呈兰色。包涵体发育至晚期破裂,宿主细胞崩解,类衣原体逸出。超微结构观察显示,包涵体内部含有大小不同的3种个体形态,即网状体、原体和中间体。网状体大而疏松,呈圆形或不规则形,大小为(890.5±164.6)nm×(623.6±129.3)nm(n=20);原体个体小而致密,呈较为整齐的短棒状或纺锤形,大小为(317.5±40.1)nm×(180.3±40.0)nm(n=18);中间体数量较少,大小介于上述2种个体之间,可能处于二者的过渡阶段。将类衣原体分离后对海湾扇贝的幼体、稚贝和成贝进行了人工感染,结果表明,类衣原体可以感染海湾扇贝不同发育阶段(幼贝、稚贝、成贝)的个体,受感染个体生长缓慢,内脏干瘪,易从附着基上脱落。但不同阶段的贝体感染率与死亡率差异较大,幼体组感染率和死亡率分别达80%和90%以上;稚贝组分别达50%和70%左右;成贝组的感染率达50%以上,但死亡率与空白组及对照组无显著差异。说明类衣原体在一定条件下可造成对幼体和稚体扇贝的危害,但对成贝影响不大,不能导致成贝宿主的大量死亡。
During a large number of deaths from October 1999 to March 2001 in the Argopectenirdidians, the histological and ultrastructural observations of the Gulf scallops at different growth stages were performed, and basophilic Chlamydia isolates were found in the shellfish glandular epithelial cells Inclusion body, the inclusion body of circular and irregular shape, the size of about 9μm × 7μm, HE staining was blue. Inclusion body development to late rupture, host cell disintegration, chlamydia escaped. Ultrastructural observation showed that inclusions contained three kinds of individual shapes with different sizes, namely, reticular bodies, protoplasts and intermediates. The reticular body was large and loose with a round or irregular shape. The size of the reticular body was (890.5 ± 164.6) nm × (623.6 ± 129.3) nm (n = 20) The spindle size was (317.5 ± 40.1) nm × (180.3 ± 40.0) nm (n = 18). The number of intermediates was small and the size was between the above two kinds of individuals, which may be in the transitional phase between them. Artificial infection of larvae, juveniles and adult scallops of Chlamydomonas isolates after the isolation of Chlamydia isolates showed that Chlamydia isolates could infect individuals at different developmental stages (juvenile, juvenile, and adult) of the Gulf scallop and infected individuals Slow, visceral shriveled, easily detached from the attachment. However, the infection rates and mortality rates of shellfish in different stages were quite different. The infection rate and mortality rate in juvenile groups were 80% and 90% respectively. The juvenile rates were 50% and 70% 50%, but the mortality rate and the blank group and the control group no significant difference. That under certain conditions, chlamydia can cause harm to larvae and juvenile scallops, but has little effect on asparagus and can not lead to a large number of deaths of catfish.