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目的探讨中性粒细胞明胶酶相关脂质运载蛋白(NGAL)在裸鼠原位胰腺癌组织中的表达情况以及与胰腺癌发生发展的关系。方法应用RT-PCR法检测22例裸鼠胰腺癌模型癌组织、癌旁组织和正常胰腺组织中NGAL基因及其编码蛋白的表达情况;采用免疫组化方法检测22例胰腺癌组织石蜡切片中NGAL蛋白表达情况。结果胰腺癌组织及其癌旁组织中NGAL m RNA相对表达量明显高于正常胰腺组织(P<0.05),胰腺癌组织中NGAL m RNA相对表达量又明显高于癌旁组织(P<0.05);NGAL蛋白在胰腺癌组织中的表达强阳性率以及相对表达量均明显高于癌旁组织和正常胰腺组织(P<0.05)。结论 NGAL在胰腺癌组织中呈高表达,NGAL可能是胰腺癌发生发展过程中的重要调节因子。
Objective To investigate the expression of neutrophil gelatinase-associated lipocalin (NGAL) in pancreatic carcinoma of the nude mouse and its relationship with the occurrence and development of pancreatic cancer. Methods The expression of NGAL gene and its coding protein in 22 pancreatic cancer tissues, adjacent normal tissues and normal pancreatic tissues were detected by RT-PCR. Immunohistochemistry was used to detect the expression of NGAL Protein expression. Results The relative expression level of NGAL mRNA in pancreatic cancer tissue and its adjacent tissues was significantly higher than that in normal pancreatic tissues (P <0.05). The relative expression level of NGAL m RNA in pancreatic cancer tissues was significantly higher than that in paracancerous tissues (P <0.05) The strong positive rate and relative expression of NGAL protein in pancreatic cancer tissues were significantly higher than those in paracancerous tissues and normal pancreatic tissues (P <0.05). Conclusion NGAL is highly expressed in pancreatic cancer tissues. NGAL may be an important regulatory factor in the development of pancreatic cancer.