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目的 :探讨醒脑静注射液 (XNJI)对脑缺血再灌注损伤 (CIRI)家兔一氧化氮 (NO)和内皮素 (ET)水平的影响。方法 :采用“四动脉闭塞法”制备家兔 CIRI模型 ,随机分为假手术对照组 (A组 )、脑缺血再灌注组(B组 )和脑缺血再灌注加 XNJI治疗组 (C组 )。分别在脑缺血前、缺血 30分钟及再灌注 30、6 0和 12 0分钟不同时间点 ,检测血浆及脑组织 NO浓度和 ET含量。结果 :脑缺血再灌注期间 ,血浆和脑组织 NO水平明显下降 ,血浆为 (43.80± 10 .4 0 )μmol/ L、(43.6 0± 8.96 )μm ol/ L、(37.5 0± 13.6 0 )μmol/ L、(39.80± 8.2 2 )μmol/ L、(40 .70± 7.86 )μmol/ L (P<0 .0 5和 P<0 .0 1) ,脑组织为 (319.0 0± 70 .70 )μmol/ g(P>0 .0 5 ) ;ET水平显著升高 ,血浆为 (73.80± 34.70 ) ng/ L、(81.30± 32 .5 0 ) ng/ L、(78.2 0± 36 .80 ) ng/ L、(10 4 .0 0± 4 2 .0 0 ) ng/ L、(111.0 0± 5 0 .70 ) ng/ L(P<0 .0 5和 P<0 .0 1) ,脑组织为 (93.10± 4 2 .30 ) ng/ g(P<0 .0 5 ) ;使用 XNJI后 ,上述各指标的异常变化明显减轻 ,与对照组相比有显著性差异 (P<0 .0 5和 P<0 .0 1)。结论 :XNJI可提高机体 NO水平、降低 ET水平而抗 CIRI
Objective: To investigate the effects of Xingnaojing injection (XNJI) on nitric oxide (NO) and endothelin (ET) levels in rabbits with cerebral ischemia-reperfusion injury (CIRI). METHODS: Rabbit CIRI models were prepared using the “four-artery occlusion method” and randomly divided into sham-operated control group (group A), cerebral ischemia-reperfusion group (group B) and cerebral ischemia-reperfusion plus XNJI treatment group (group C). ). The levels of NO and ET in plasma and brain tissue were measured before cerebral ischemia, 30 minutes after ischemia, and at 30, 60, and 120 minutes after reperfusion. RESULTS: During cerebral ischemia-reperfusion period, plasma and brain NO levels were significantly decreased. Plasma levels were (43.80 ± 10.4) μmol/L, (43.6 0 ± 8.96) μmol/L, (37.5 0 ± 13.6 0). Μmol/L, (39.80±8.22) μmol/L, (40.70±7.86) μmol/L (P<0.05 and P<0.01), and brain tissue (319.0 ± 70.70) ) μmol / g (P> 0.05); ET levels increased significantly, plasma (73.80 ± 34.70) ng / L, (81.30 ± 32.50) ng / L, (78.2 0 ± 36.80) Ng/L, (10 4 .0 0 4 2 .0 0) ng/L, (111.0 0± 5 0 .70) ng/L (P<0.05 and P<0.01). The tissue was (93.10± 42.30) ng/g (P<0.05). After using XNJI, the abnormal changes of the above indexes were significantly reduced, compared with the control group, there was a significant difference (P<0. 0 5 and P<0. 0 1). Conclusion : XNJI can increase NO level in the body, reduce ET levels and resist CIRI