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目的:建立测定旱莲胶囊中补骨脂素和异补骨脂素含量的高效液相色谱方法。方法:色谱柱为Thermo C_(18)柱(250 mm×4.6 mm,5μm),流动相为甲醇-水(50:50),流速为0.6 ml·min~(-1),检测波长为295 nm。结果:补骨脂素的线性范围为0.2～0.6μg,r=0.999 8(n=5),平均回收率为99.2%,RSD为1.01%;异补骨脂素的线性范围为0.2～0.6μg,r=0.999 9(n =5),平均回收率为100.3%,RSD为2.22%。结论:该方法操作简便,准确,精密度高,可用于控制旱莲胶囊的质量。
Objective: To establish a high performance liquid chromatography method for the determination of psoralen and isopsoralen in the Erythrasian capsules. METHODS: The column was a Thermo C 18 column (250 mm×4.6 mm, 5 μm). The mobile phase was methanol-water (50:50). The flow rate was 0.6 ml·min -1 and the detection wavelength was 295 nm. . Results: The linear range of psoralen was 0.2-0.6 μg, r=0.999 8 (n=5). The average recovery was 99.2% and the RSD was 1.01%. The linear range of isopsoralen was 0.2-0.6 μg. , r=0.999 9 (n = 5), the average recovery was 100.3%, and the RSD was 2.22%. Conclusion: This method is simple, accurate and high in precision, and can be used to control the quality of the dry lotus capsules.