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目的 探讨胃癌组织中p5 3与血管内皮生长因子 (VEGF)表达和血管生成的关系。方法 应用免疫组织化学技术对 6 0例胃癌组织进行p5 3、VEGF表达和肿瘤组织微血管密度MVD检测。结果 MVD以及p5 3和VEGF的表达与胃癌病灶的大小 (P <0 0 5 )、浸润深度 (P <0 0 1)、淋巴结转移 (P <0 0 1)和TNM分期 (P <0 0 1)密切相关。p5 3的表达与VEGF的表达显著相关 (χ2 =2 9 5 1,P <0 0 1) ;且p5 3和VEGF均阳性的胃癌组织MVD明显高于两者均阴性者 (P <0 0 1)。生存分析显示 :死亡组的平均MVD〔(5 0± 19)个 /视野〕显著高于存活者〔(2 7± 6 )个 /视野〕(P <0 0 1)。多元回归分析表明MVD是影响预后的独立因素。结论 胃癌组织突变的p5 3可上调VEGF的表达 ,促进血管生成 ,进而影响胃癌的进展
Objective To investigate the relationship between p53 and vascular endothelial growth factor (VEGF) expression and angiogenesis in gastric cancer. Methods Immunohistochemistry was used to detect the expression of p53, VEGF and microvessel density (MVD) in 60 cases of gastric cancer. Results The expressions of MVD and p53 and VEGF were related to the size of gastric cancer lesions (P < 0.05), depth of invasion (P <0 01), lymph node metastasis (P <0 01) and TNM stage (P <0 01. )closely related. The expression of p53 was significantly correlated with the expression of VEGF (χ2 = 2 951, P <0 01). The MVD of gastric cancer tissues with positive p53 and VEGF were significantly higher than those with negative VEGF (P <0 01). ). Survival analysis showed that the mean MVD ((50±19)/fields) in the death group was significantly higher than that in the survivors ((2 7±6)/fields) (P < 0 01). Multiple regression analysis showed that MVD was an independent factor that affected the prognosis. Conclusion The mutation of p53 in gastric cancer can upregulate the expression of VEGF, promote angiogenesis, and influence the progression of gastric cancer.