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目的探讨脑肿瘤ｐ５３蛋白表达状况对其细胞增殖和凋亡的影响，及这些指标与脑肿瘤组织学类型和恶性程度的关系。方法对１０例对照脑组织和８０例脑肿瘤标本进行原位细胞凋亡及免疫组化标记。结果６９例脑肿瘤（８６．３％）表达ｐ５３蛋白，阳性细胞含量随肿瘤恶性程度升高而增加，对照组全部阴性。各肿瘤组增殖细胞核抗原和Ｋｉ－６７抗原阳性细胞密度均高于对照组，并随肿瘤恶性程度及ｐ５３蛋白表达升高而增加，凋亡细胞密度均低于对照组，并随肿瘤恶性程度及ｐ５３蛋白表达升高而降低。结论提示以上４种指标对评价脑肿瘤生物学行为有参考价值，脑肿瘤ｐ５３蛋白表达和功能异常与其细胞增殖及凋亡失衡有关，可能是原发性和转移性脑肿瘤发生发展的重要因素。
Objective To investigate the effect of p53 protein expression on cell proliferation and apoptosis in brain tumors and their relationship with the histological type and malignancy of brain tumors. Methods 10 cases of control brain tissue and 80 cases of brain tumor specimens were in situ apoptosis and immunohistochemical markers. Results 69 cases of brain tumor (86.3%) expressed p53 protein. The content of positive cells increased with the malignant degree of the tumor, while the control group was all negative. The density of proliferating cell nuclear antigen and Ki-67 positive cell in each tumor group were higher than those in control group, and increased with the malignant degree and p53 protein expression. The density of apoptotic cells in control group was lower than that in control group, p53 protein expression increased and decreased. Conclusions These results suggest that the above four indexes are valuable for evaluating the biological behavior of brain tumors. The expression and dysfunction of p53 protein in brain tumors are related to cell proliferation and imbalance of apoptosis, which may be an important factor in the development of primary and metastatic brain tumors.