ABC试剂,一般在染色前30分钟配好,放冰箱备用。我们在进行肝癌免疫组化研究中,试用了两个月和半年前配好的ABC试剂,并与提前30分钟配制的ABC试剂进行了比较。结果发现新、旧试剂没有明显区别,均获满意结果。因此、在配制ABC试剂时,不必再计算切片的数量、可一次配制5～10ml,每次染色直接滴加,直到用完为止(半年内)。省去每次染 ABC reagents are generally prepared 30 minutes before staining and placed in a refrigerator. In the immunohistochemical study of hepatocellular carcinoma, we tested the ABC reagents that were prepared two months and six months ago and compared them with ABC reagents that were prepared 30 minutes earlier. As a result, there was no significant difference between the new and old reagents, and satisfactory results were obtained. Therefore, in the preparation of ABC reagents, it is not necessary to calculate the number of sections, can be prepared at a time 5-10ml, each directly to the dyeing, until run out (in six months). Save every time