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淋巴毒素(LT)在体外能溶解各种靶细胞,可作为细胞介导免疫介质参与组织破坏反应。干扰素(IFN)有调节淋巴细胞在体外破坏靶细胞的作用。IFN不仅能提高NK细胞的溶解率,而且也能增加NK细胞的循环。最近有人报告,IFN是一种合成蛋白质,它能保护靶细胞。由于IFN对LT诱导靶细胞的损伤有重要的调变作用,所以探讨IFN增强LT诱导靶细胞损伤的作用是有意义的。本文试验用的靶细胞为Hela细胞和WI-38细胞,IFN为人的淋巴细胞所产生的IFN-α及用DNA重组技术由大肠杆菌产生的人IFN-α、LT用从人扁桃体分离的淋巴细胞加PHA-P培养的上清液。LT试验是用形态学和~3H胸腺嘧啶测定细胞活力的方法。作者获得的实验结果如下: (1)Hela细胞加各种剂量的IFN-α预先温育24小时后洗涤,再加入不同稀释度
Lymphotoxin (LT) can dissolve various target cells in vitro, and can serve as a cell-mediated immune mediator to participate in the tissue destruction reaction. Interferon (IFN) regulates lymphocyte destruction of target cells in vitro. IFN not only increases the rate of NK cell lysis but also increases the circulation of NK cells. It has recently been reported that IFN is a synthetic protein that protects target cells. Since IFN has an important modulatory effect on LT-induced damage of target cells, it is of interest to explore the role of IFN in enhancing LT-induced target cell injury. The target cells used in this study were Hela cells and WI-38 cells, IFN-α produced by human lymphocytes of IFN and human IFN-α produced by Escherichia coli using DNA recombination technology. LT was isolated from human tonsil lymphocytes Add supernatant of PHA-P culture. The LT test is a measure of cell viability using morphology and ~ 3H thymidine. The experimental results obtained by the authors are as follows: (1) Hela cells were pre-incubated with various doses of IFN-α for 24 hours and then washed, then added with different dilutions