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目的:探索中成药中非法添加化学成分的通用检测方法并论证建立DAD光谱库的重要性。方法:采用ShimadzuShim-Pack VP-ODS(250 mm×4.6 mm,5μm)色谱柱,采用梯度洗脱,以二极管阵列检测器检测并对检出色谱峰的光谱图与光谱库中的光谱图比较,粗筛出非法添加的化学成分,并对检出的化学成分采用LC-MS进行验证。结果:可以检出吲哚美辛、吡罗昔康、醋酸泼尼松。结论:采用DAD光谱库可大大缩短筛选的时间,提高工作效率。
OBJECTIVE: To explore the universal testing method of illegally added chemical components in Chinese patent medicines and demonstrate the importance of establishing DAD spectral libraries. Methods: A Shimadzu Shim-Pack VP-ODS (250 mm × 4.6 mm, 5 μm) column was used for gradient elution. The diode array detector was used to detect and compare the spectra of the detected peaks with the spectra in the spectral library. The chemicals added illegally were screened out, and the chemical constituents detected were verified by LC-MS. Results: Indomethacin, piroxicam, prednisone acetate can be detected. Conclusion: DAD spectral library can greatly shorten the screening time, improve work efficiency.