论文部分内容阅读
将烟草中经修饰的Ⅰ类几了质酶和β－1，3-葡聚糖酶cDNA分别加上CaMV35S启动子和NOS终止子，依次插入到同一植物表达载体pBin19上，获得植物双价表达载体pCG－Ⅱ。然后载体pCG－Ⅱ以土壤农杆菌LBA4404介导转化番茄，再生植株的点杂交及PCR扩增证明两种cDNA已随T－DNA同时导入番茄植株中。
The tobacco plants modified class I endoplasmic enzymes and β-1,3-glucanase cDNA were added CaMV35S promoter and NOS terminator, followed by insertion into the same plant expression vector pBin19 to obtain the plant bivalent expression Vector pCG-II. Then the vector pCG-Ⅱ was transformed into tomato by Agrobacterium tumefaciens LBA4404. The spot hybridization and PCR amplification of the regenerated plants proved that both cDNAs had been introduced into tomato plants simultaneously with T-DNA.