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本文报告“霍乱一步法快诊试剂”在国内部分地区现场应用及与常规分离鉴定法的比较结果。共观察了 79份粪便标本直接使用“一步法快诊”的检测结果及 47份标本增菌 6 - 8小时后同样用该法的检测结果。现场考查表明 ,一步快诊法适用于检查急性期病人 ,从粪便标本中不仅能查出活菌 ,还能检查就诊前服用过抗菌药物的患者 ,其检出率高于常规培养法 (χ2 =17 2 5P <0 0 0 5 ) ;而增菌后标本的一步快诊法能得到和常规法完全相同的特异性与敏感性 ,可用于检定轻型病人和健康带菌者 ,但比常规法简便、快速。
This article reports the “Cholera one-step quick-diagnosis reagent” in some parts of the domestic field applications and compared with the conventional separation and identification results. A total of 79 stool specimens were observed using the “one-step quick diagnosis” test results and 47 samples of bacteria by 6-8 hours after the same method with the test results. The field test showed that the one-step quick diagnosis method is suitable for the examination of patients in acute stage. Not only the viable bacteria can be detected from the stool specimens, but also the patients taking anti-bacterial drugs before the treatment, the detection rate is higher than that of the conventional culture method (χ2 = 17 2 5P <0 0 0 5). However, the one-step quick-diagnosis method of the sample after enrichment can obtain exactly the same specificity and sensitivity as the conventional method, and can be used to test the light-weight patients and healthy carriers. Compared with the conventional method, fast.