金边瑞香(Daphne odora var. marginata)是名贵常绿观叶、观花植物,为瑞香属中的上品,是我国园林传统珍贵稀有花木。除观赏外,还有较高的经济和药用价值。由于其资源在国内比较稀少,用常规无性繁殖方法速度慢,而利用组织培养方法,对加快金边瑞香的无性繁殖,具有一定的意义。 一、材料和培养条件 取多年生植株当年抽出的幼嫩枝条上的顶芽和带芽腋茎段,用常规方法表面消毒后,在无菌条件下剥去幼嫩顶芽的外层叶片,茎段切成带有1—2个叶痕的小段,接种于MS附加有不同浓度BA、IAA,IBA等成分的培养基上,在温度20—25℃,光照强度1000Lux,每天照光8—10小时条件下进行培养。 二、分化与生长情况 将金边瑞香的顶芽和茎段接种于MS附 Daphne odora var. Marginata is a valuable evergreen foliage, ornamental plants, is the top grade in the genus Rumex and is a precious and precious traditional Chinese garden flower. In addition to watching, there are higher economic and medicinal value. Due to its relatively scarce resources in China, the conventional method of vegetative propagation is slow, and the use of tissue culture methods is of great significance to speed up the clonal propagation of Daphne victualana. First, the material and culture conditions Take the perennial plant that year out of the young shoots on the top buds and shoots axillary stems section, using conventional methods of surface disinfection, stripped under sterile conditions outer buds of the top outer leaves, stems The segments were cut into small segments with 1-2 leaf marks and inoculated on medium supplemented with different concentrations of BA, IAA, IBA and other components at a temperature of 20-25 ° C with a light intensity of 1000 Lux and 8-10 hours of light per day Under the conditions of culture. Second, the differentiation and growth of Phnom Penh Ruixiang the top buds and stems inoculated MS attached