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目的:探讨长程多柔比星(adriamycin,ADR)作用乳腺癌细胞株MCF-7对富集肿瘤干细胞的可能性。方法:采用长程ADR诱导的方法建立ADR耐药细胞株MCF-7/ADR’。ALDEFLUOR法检测亲本MCF-7细胞及ADR耐药细胞MCF-7/ADR’中乙醛脱氢酶1(aldehyde dehydrogenase 1,ALDH1)阳性的肿瘤干细胞亚群的比例,然后采用无血清悬浮培养和裸鼠成瘤实验分别检测两者在体外形成干细胞微球体和体内成瘤能力的差异。结果:MCF-7和MCF-7/ADR’细胞中ALDH1+肿瘤干细胞亚群的比例分别为(0.82±0.77)%和(8.21±2.38)%,两者差异有统计学意义(P<0.05);两者经无血清悬浮培养形成干细胞微球体的比例分别为(2.17±0.70)%和(7.87±1.39)%,差异有统计学意义(P<0.05)。MCF-7/ADR’细胞在裸鼠体内的成瘤能力明显强于MCF-7细胞。结论:长程ADR作用MCF-7后可富集肿瘤干细胞。
Objective: To investigate the possibility of breast cancer cell line MCF-7 on the enrichment of tumor stem cells by adriamycin (ADR). Methods: ADR resistant cell line MCF-7 / ADR ’was established by long-term ADR induction. ALDEFLUOR method was used to detect the ratio of ALDH1-positive tumor stem cell subsets in MCF-7 cells and ADR-resistant cells MCF-7 / ADR ’, and then cultured in serum-free suspension culture and nude Tumorigenicity experiments were used to detect the differences of the two in vitro forming stem cell microspheres and in vivo tumorigenicity. Results: The percentage of ALDH1 + tumor stem cell subsets in MCF-7 and MCF-7 / ADR ’cells were (0.82 ± 0.77)% and (8.21 ± 2.38)%, respectively, with statistical significance (P <0.05). The percentage of stem cell microspheres cultured in serum-free suspension was (2.17 ± 0.70)% and (7.87 ± 1.39)%, respectively, with statistical significance (P <0.05). The tumorigenicity of MCF-7 / ADR ’cells in nude mice was significantly stronger than that of MCF-7 cells. Conclusion: Long-term ADR can enrich tumor stem cells after MCF-7 treatment.