应用RT PCR一步法检测了 42例慢性粒细胞白血病 (CML)患者的bcr/abl融合基因 ,均显示bcr/abl基因阳性 ,其中2 1例为 16 8bp扩增带 ,18例为 93bp扩增带 ,3例同时具有 16 8bp和 93bp二条扩增带。bcr/abl融合基因检测 ,对CML的诊断、治疗及预后判断均有重要意义。RT PCR一步法特异性好 ,敏感性高 ,简单快速 ,可节省试剂 ,值得推广应用。 One-step RT PCR was used to detect bcr/abl fusion genes in 42 patients with chronic myeloid leukemia (CML). All of them showed positive bcr/abl genes, of which 16 were amplified by 216 bp and 18 bp by 93 bp. In 3 cases, there were two amplification bands of 16 8bp and 93bp. The detection of bcr/abl fusion gene has important significance for the diagnosis, treatment and prognosis of CML. One-step RT PCR method has good specificity, high sensitivity, simple and rapid, and can save reagents. It is worth popularizing and applying.