论文部分内容阅读
2006年夏季,本课题组在新疆喀什地区伽师县库蚊标本中分离到我国首株Tahyna病毒XJ0625株,并发现当地不明原因发热患者存在该病毒感染。本研究通过细胞培养、动物实验、电镜观察、间接免疫荧光及交叉保护中和试验等研究对XJ0625病毒株的细胞易感性、动物致病性、形态学及抗原性等特征进行观察,并应用分子生物学软件对其分子进化特征加以分析。结果发现该毒株可以引起BHK-21细胞病变,乳鼠颅内接种该毒株可以引起死亡。与其他布尼亚病毒形态相似,Tahyna病毒为球形有包膜病毒。该病毒与国际流行Tahyna病毒Bardos92株的抗体作用,在间接免疫荧光试验中呈现阳性荧光信号。空斑减少中和试验结果显示该抗体对XJ0625病毒株的中和效价为1∶3 200。核苷酸序列分析显示,该病毒与Bardos92株处于同一个进化分支,二者S节段同源性为91.8%,M节段同源性为81.9%。
In the summer of 2006, our group isolated the first strain of Tahyna virus XJ0625 from Cuijia County, Kashi Prefecture, Xinjiang Uygur Autonomous Region, and found that the virus was found in the local unexplained fever. In this study, cell susceptibility, animal pathogenicity, morphology and antigenicity of XJ0625 strain were observed by cell culture, animal experiments, electron microscopy, indirect immunofluorescence and cross-protection neutralization test. Biology software analyzes its molecular evolutionary characteristics. The results showed that the strain can cause BHK-21 cell lesions, intracerebral inoculation of the strain can cause death. Like other Bunyan viruses, Tahyna virus is a globular enveloped virus. The virus and the international epidemic Tahyna virus Bardos92 strain of antibodies, indirect immunofluorescence showed positive fluorescence signal. Plaque reduction and neutralization test results showed that the neutralizing titer of the antibody to XJ0625 strain was 1: 300. Nucleotide sequence analysis showed that the virus was in the same evolutionary branch as Bardos92, with 91.8% identity in S segment and 81.9% in M segment.