论文部分内容阅读
ASR(ABA,stress,ripening induced protein)是一类响应植物干旱胁迫的关键转录因子,在许多植物中已有报道,然而尚未见香蕉(Musa acuminata)中ASR与抗旱作用的相关研究。该实验从香蕉果实cDNA文库中筛选出1个ASR基因,即MaASR1(登录号为AY628102)。干旱胁迫下,该基因在叶片中的表达量高于根部。将MaASR1转入拟南芥(Arabidopsis thaliana),Southern检测确定了两株独立表达的转基因株系(命名为L14和L38)。表型观察发现,此两转基因株系的叶片变小且变厚;Northern和Western检测结果表明,MaASR1在L14和L38中表达。控水处理后,L14和L38的存活率及脯氨酸含量均高于野生型。经干旱胁迫和外源ABA处理后,对MaASR1转基因株系中ABA/胁迫响应基因的表达分析,发现MaASR1可增强转基因株系对ABA信号的敏感度,但不能增强植株依赖于ABA途径的抗旱性。
Stress-induced ripening induced protein (ASR), a key transcription factor responding to drought stress in plants, has been reported in many plants. However, no studies have been done on the association between ASR and drought resistance in banana (Musa acuminata). This experiment screened a ASR gene from banana fruit cDNA library, namely MaASR1 (Accession No. AY628102). Under drought stress, the expression level of this gene in leaves was higher than that in roots. MaASR1 was transferred into Arabidopsis thaliana and Southern assays identified two independently expressed transgenic lines (designated L14 and L38). Phenotypic analysis showed that leaves of both transgenic lines became smaller and thicker. Northern and Western blotting showed that MaASR1 was expressed in L14 and L38. Controlled water treatment, L14 and L38 survival rate and proline content were higher than the wild type. After drought stress and exogenous ABA treatment, the expression of ABA / stress response genes in MaASR1 transgenic lines was analyzed and found that MaASR1 enhanced the sensitivity of transgenic lines to ABA signaling but did not enhance the drought resistance of plants dependent on ABA pathway .