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研究了细胞质膜钙通道阻断剂氯化镧(LaCl3)、胞内IP3通道阻断剂肝素(Heparin)、胞内CaM活性抑制剂三氟啦嗪(TFP)对长春花光合作用的影响,结果发现:Ca2+通道阻断剂处理后长春花叶片净光合速率(Pn)、PSII最大量子产量(Fv/Fm)、PSⅡ实际量子产量(Yield)、电子传递速率(ETR)、光化学淬灭系数(qP)均下降,非光化学淬灭系数(qN)及Chla、Chlb、Chla+Chlb含量上升,表明Ca2+通道阻断剂对长春花的光合作用有较大影响,且不是通过加速叶片光合色素降解或抑制其合成来实现抑制叶片的光合能力;其中Heparin处理的长春花叶片相关参数变化幅度最大,表明胞内钙库通过IP3通道释放的Ca2+在长春花叶片光合作用过程中发挥了更积极的作用.
The effects of LaCl3, Heparin, an intracellular CaM inhibitor, and trifluoperazine (TFP), an inhibitor of intracellular CaM, on the photosynthesis of Catharanthus roseus were studied. Results The results showed that the net photosynthetic rate (Pn), PSII maximum quantum yield (Fv / Fm), PSII actual yield, electron transfer rate (ETR) and photochemical quenching coefficient (qP ) Decreased, the non-photochemical quenching coefficient (qN) and the content of Chla, Chlb and Chla + Chlb increased, which indicated that the Ca2 + channel blocker had a significant effect on the photosynthesis of P. vinifera and did not accelerate the photosynthetic pigment degradation or inhibition Which was synthesized to inhibit the photosynthetic capacity of leaves. Among them, Heparin-treated leaves had the largest change in the related parameters of vinca virescens leaves, indicating that Ca2 + released by intracellular calcium stores through IP3 channels played a more active role in photosynthesis of vinca virescens leaves.