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建立了选择性测定血浆中普罗帕酮对映异构体的柱前衍生化反相ＨＰＬＣ法。用Ｓ（＋）１（１萘基）乙基异氰酸酯为衍生化试剂，与血浆中提取出的普罗帕酮反应生成非对映立体异构体，以ＨＰＬＣＵＶ检测法（２２０ｎｍ）定量。采用此法成功地测试了１０名健康受试者单剂量口服３００ｍｇ盐酸普罗帕酮片后对映异构体的药代动力学曲线。此法灵敏度高（７５ｎｇ·ｍｌ－１），操作简便，重现性好。
A pre-column derivatization reversed-phase HPLC method was developed for the selective determination of propafenone enantiomers in plasma. Diastereoisomers were obtained by reaction with propafenone extracted from plasma using S (+) 1 (1naphthyl) ethyl isocyanate as the derivatization reagent. The HPLCUV detection (220 nm) Quantitative. Using this method, the pharmacokinetic profiles of the enantiomers of a single oral dose of 300 mg propafenone hydrochloride tablets in 10 healthy subjects were successfully tested. This method is highly sensitive (7 5ng · ml-1), easy to operate, reproducible.