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建立了选择性测定血浆中普罗帕酮对映异构体的柱前衍生化反相HPLC法。用S(+)1(1萘基)乙基异氰酸酯为衍生化试剂,与血浆中提取出的普罗帕酮反应生成非对映立体异构体,以HPLCUV检测法(220nm)定量。采用此法成功地测试了10名健康受试者单剂量口服300mg盐酸普罗帕酮片后对映异构体的药代动力学曲线。此法灵敏度高(75ng·ml-1),操作简便,重现性好。
A pre-column derivatization reversed-phase HPLC method was developed for the selective determination of propafenone enantiomers in plasma. Diastereoisomers were obtained by reaction with propafenone extracted from plasma using S (+) 1 (1naphthyl) ethyl isocyanate as the derivatization reagent. The HPLCUV detection (220 nm) Quantitative. Using this method, the pharmacokinetic profiles of the enantiomers of a single oral dose of 300 mg propafenone hydrochloride tablets in 10 healthy subjects were successfully tested. This method is highly sensitive (7 5ng · ml-1), easy to operate, reproducible.