论文部分内容阅读
目的:观察樱桃花青素对佐剂性关节炎(AA)大鼠自由基和抗氧化功能以及炎症因子的影响。方法:将50只SD雄性大鼠随机分为5组即正常组、模型组、樱桃花青素高、中、低剂量组。对足爪肿胀进行测量,在光镜下观察各组组织形态学变化,用比色法检测了动物外周全血的谷胱甘肽过氧化物酶(GSH-PX)活性、血清超氧化物酶(SOD)活性、血清丙二醛(MDA)含量以及血清总抗氧化能力(T-AOC)。用ELISA检测血清中TNF-α含量,用放射免疫法检测了足爪PGE2含量。结果:模型组和正常组相比GSH-PX,SOD活性和T-AOC能力下降,血清MDA含量升高,血清中TNF-α和足爪PGE2含量升高。樱桃花青素组能升高GSH-PX,SOD和T-AOC活力,降低血清MDA,TNF-α以及足爪PGE2含量。光镜下组织形态观察表明樱桃花青素各组能不同程度减轻滑膜增生,减少炎细胞浸润。结论:樱桃花青素能增强AA抗氧化能力,并能降低炎症细胞因子PGE2和TNF-α,从而减轻AA模型的关节炎损伤。
OBJECTIVE: To observe the effects of cherry anthocyanins on free radicals, antioxidative functions and inflammatory factors in adjuvant arthritis (AA) rats. METHODS: Fifty SD male rats were randomly divided into five groups: the normal group, the model group, and the cherry high, medium, and low dose group. Foot edema was measured and histomorphological changes were observed under a light microscope. Glutathione peroxidase (GSH-PX) activity and serum superoxide enzymes were measured by colorimetry. (SOD) activity, serum malondialdehyde (MDA) content, and serum total antioxidant capacity (T-AOC). The content of TNF-α in serum was detected by ELISA. The content of PGE2 in paws was detected by radioimmunoassay. Results: Compared with the normal group, the GSH-PX, SOD activity and T-AOC ability of the model group were decreased, serum MDA content was increased, serum TNF-α and paw PGE2 content were increased. The cherry anthocyanin group can increase the activity of GSH-PX, SOD and T-AOC, and decrease the content of serum MDA, TNF-α and paw PGE2. Under light microscope, the histomorphological observation showed that cherry anthocyanins could reduce synoviocyte proliferation and reduce inflammatory cell infiltration in different degrees. Conclusion: Anthocyanin can enhance the antioxidant capacity of AA and reduce the inflammatory cytokines PGE2 and TNF-α, thus reducing the arthritis injury of AA model.