花色苷是调控果实着色的主要色素之一,具有多种保健功能。为探索花色苷在乌桃果实成熟过程中的降解与果胶裂解酶的关系,利用已报到的碧桃果胶裂解酶基因序列设计特异引物,从乌桃中克隆出果胶裂解酶基因PpPL,PpPL包括1239bp的开放阅读框,编码413个氨基酸。利用荧光定量PCR检测PpPL在果实不同成熟度及不同组织中的表达,数据显示,乌桃PpPL基因的表达量随着果实的成熟及花色苷的降解呈现递增的趋势,且在果实成熟末期表达量明显增多;不同组织中PpPL基因均有表达,同一成熟度,其相对表达量从少到多依次为:幼果果肉<叶<幼果果皮<茎,推测PpPL可能参与花色苷的降解。 Anthocyanins are one of the main pigments that regulate the coloring of fruits and have a variety of health care functions. In order to explore the relationship between the degradation of pectin lyase and the degradation of anthocyanins during the ripening process of peach fruit, the pectin lyase genes PpPL and PpPL Including 1239bp open reading frame, encoding 413 amino acids. The fluorescence quantitative PCR was used to detect the expression of PpPL in different maturity and different tissues of fruit. The data showed that the expression of PpPL gene showed an increasing trend with the maturation of fruit and the degradation of anthocyanins, and the expression of PpPL at the end of fruit ripening PpPL gene in different tissues were expressed at the same maturity. The relative expression levels of PpPL gene in different tissues were as follows: young fruit pulp