Webster等推测,人类免疫缺陷症病毒(HIV即HTLV-Ⅲ/LAV)可残存于生产Cohn组分Ⅱ材料的乙醇沉淀物中,但也有人证实通过Cohn-Oncley分级分离法的组分Ⅱ或滤液Ⅲ(pH 4.0)可去除其中的HIV。本文作者观察了对具有免疫活性的个体给予静注免疫球蛋白(IGIV)后血清抗-HIV阳转的可能性。作者所用经过还原和烷化的IGIV,是从组分Ⅱ中分离出来的;未经还原和烷化的IGIV是从pH 4的滤液Ⅲ中分离的。最后的制品还要经过一个培养步骤,此步骤能使感染颗粒再降低3～4个对数值。 Webster et al. Hypothesized that human immunodeficiency virus (HIV), HTLV-III / LAV, may remain in the ethanol precipitate that produces Cohn fraction II material, but it has also been shown that fraction II or filtrate by Cohn-Oncley fractionation Ⅲ (pH 4.0) to remove the HIV. The authors observed the possibility of seroconversion of anti-HIV after intravenous immunoglobulin (IGIV) in immunocompetent individuals. The reduced and alkylated IGIV used by the authors was isolated from component II; unreduced and alkylated IGIV was isolated from filtrate III at pH 4. The final product is also subjected to a culture step, which reduces the number of infected particles by 3 to 4 log.