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椎间盘退变(Degenerative disc disease,DDD)是下腰痛的主要原因。当前临床治疗主要目的在于恢复生物力学功能和缓解症状。而椎间盘组织工程的兴起为DDD的治疗提供了新方法,其中干细胞是用于椎间盘组织工程的重要种子细胞。在本实验中,我们研究人骨髓间充质干细胞(MSCs)体外分离培养的细胞学特性以及在Pellet三维培养体系下构建椎间盘组织工程细胞的可行性。结果提示该方法培养的MSCs具较高的纯度,且体外培养具有较强的增殖能力,短时间内可大量扩增,达到组织工程需要的种子细胞数量要求,且细胞保持未分化状态,具有良好的生物安全性。并能表达Ⅱ型胶原和蛋白多糖等椎间盘细胞外基质,因此是椎间盘组织工程理想的种子细胞。
Degenerative disc disease (DDD) is the main cause of low back pain. The main purpose of current clinical treatment is to restore biomechanical function and relieve symptoms. The rise of disc tissue engineering provides a new approach to the treatment of DDD, in which stem cells are important seed cells for disc tissue engineering. In this experiment, we investigated the cytological characteristics of human bone marrow mesenchymal stem cells (MSCs) isolated and cultured in vitro and the feasibility of constructing tissue engineering cells under the Pellet three-dimensional culture system. The results suggest that MSCs cultured by this method have high purity, and have strong proliferative capacity in vitro, which can be expanded in a short time, reach the required number of seed cells required for tissue engineering, and the cells remain in an undifferentiated state with good Biosecurity. And can express type Ⅱ collagen and proteoglycans and other extracellular matrix of disc, it is the ideal tissue engineering seed cells.