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已知谷酰胺是一非必需氨基酸,但对肠道是一不可缺少的营养物,它可刺激体内肠粘膜的生长,有关其机制尚不清楚.作者的实验旨在阐明谷酰胺在上皮生长因子(EGF)刺激肠细胞增生中的作用,并明确EGF的阿种特殊有丝分裂作用需要谷酰胺.用EGF(20ng/ml)刺激未转化的鼠肠粘膜细胞系(IEC-6),不加或加入谷酰胺(0.1～10mmol/L).分别用掺入氚化胸腺嘧啶核甙、氚化尿核甙和~(14)C一亮氨酸定量测定DNA、RNA、蛋白质合成,对早期生
Glutamine is known to be a nonessential amino acid, but an essential nutrient in the gut, which stimulates the growth of intestinal mucosa in vivo. The mechanism by which glutamine is not known remains unclear. The authors’ experiments aimed to elucidate the effect of glutamine on epithelial growth factor (EGF) to stimulate intestinal cell proliferation and to clarify that the specific mitosis of EGF is required for glutamine. The untransformed murine intestinal mucosal cell line (IEC-6) was stimulated with EGF (20 ng / ml) without or with Glutamine (0.1 ~ 10mmol / L) .Thymosin, tritiated uridine and ~ (14) C-leucine were respectively used for the quantitative determination of DNA, RNA and protein synthesis,