“利用PCR技术来扩增目的基因”是人教版高中生物(选修3)《现代生物科学技术专题》中基因工程操作步骤中获取目的基因的常用方法之一,是基因工程操作中的重点难点,也是命题的热点,其内容抽象,难以理解,让很多学生望而生畏。故此,笔者想和学生一起动动手,揭开其神秘的面纱。一、对PCR技术进行动手模拟的必要性分析关于PCR技术,常规的教学方法就是先回顾DNA体内复制的过程和条件,再结合教材带领学 “The use of PCR to amplify a gene of interest” is one of the commonly used methods for obtaining a gene of interest in genetic engineering procedures in the PEP Biology (Elective 3) “Modern Bioscience and Technology Program”. It is one of the common methods used in genetic engineering The key difficulties, but also the hot topic proposition, its content is abstract, difficult to understand, so many students daunting. Therefore, I want to move hands with students, opened its mysterious veil. First, the necessity of hands-on simulation of PCR technology PCR technology, the conventional teaching method is to first review the process and conditions of DNA replication in vivo, combined with the teaching material led the study