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根据五节芒(Miscanthus floridulus)定居盖度(0、17%、41%、71%和89%),选择了5个尾矿砂研究样地,并分析了尾矿砂微生物群落结构动态及其与土壤理化特性、重金属含量和植物生物量的关系。结果表明:随着五节芒在尾矿砂上的定居,除pH以外,尾矿砂的有机碳、总氮、总磷、NH4+-N、NO3--N、速效磷的含量、团聚体稳定性和最大持水量均显著性提高,而土壤重金属总量与DTPA可提取量均显著性下降。主分量分析表明,随着五节芒定居,尾矿砂微生物群落总体结构发生了显著变化,其中革兰氏阳性细菌、革兰氏阴性细菌、真菌、放线菌、菌根菌(16∶1ω5c和20∶3ω3)、藻类脂肪酸含量以及微生物群落多样性(香农-维纳指数)均显著提高,并且与一些土壤理化特性,如NO3--N、速效磷、团聚体稳定性、土壤全氮和五节芒地上、地下生物量存在着显著或极显著正相关关系。仅有革兰氏阴性细菌和菌根菌脂肪酸含量分别与DTPA-铜呈显著负相关。
According to the habitat cover of Miscanthus floridulus (0, 17%, 41%, 71% and 89%), five tailing sand samples were selected to study the structure of tailings sand microbial community and its relationship with soil Physicochemical properties, heavy metal content and plant biomass. The results showed that the content of organic carbon, total nitrogen, total phosphorus, NH4 + -N, NO3 - N, available phosphorus in the tailing sand, the stability of aggregate and The maximum water holding capacity increased significantly, while the total amount of soil heavy metals and extractable DTPA decreased significantly. The principal component analysis showed that the overall structure of the tailings sand microbial communities changed significantly with the development of five mulches, including Gram-positive bacteria, Gram-negative bacteria, fungi, actinomycetes, mycorrhizal bacteria 20:3ω3), algae fatty acid content and microbial community diversity (Shannon-Wiener index) were significantly increased, and with some soil physical and chemical properties, such as NO3 - N, available phosphorus, stability of aggregates, soil total nitrogen and five There was a significant or very significant positive correlation between subsurface biomass and aboveground biomass. Only Gram-negative bacteria and mycorrhizal bacteria fatty acid content were significantly negatively correlated with DTPA-copper.