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目的:通过研究γ60Co-射线辐照与模拟微重力效应对大鼠终末分化PC12细胞的损伤,分析空间环境对哺乳动物细胞的损伤。方法:利用大鼠终末分化PC12细胞为材料,通过检测地面对照组、γ~(60)Co-射线辐照组、模拟微重力效应对照组、γ~(60)Co-射线辐照复合模拟微重力效应组的微核率、微核细胞率及HPRT基因突变频率,分析模拟空间环境对哺乳动物细胞的损伤。结果:随着γ~(60)Co-射线辐照剂量的增加,微核率、微核细胞率及HPRT基因突变频率均增加,呈现比较良好的效应关系,而γ~(60)Co-射线辐照复合模拟微重力效应组各项指标均低于γ~(60)Co-射线辐照组。结论:γ~(60)Co-射线辐照及模拟微重力效应均能诱发大鼠终末分化PC12细胞染色体的损伤和HPRT基因的突变,而模拟微重力效应降低PC12细胞损伤的机制还有待进一步研究。
OBJECTIVE: To study the damage of terminal differentiation PC12 cells by γ60Co-ray irradiation and simulated microgravity effects in mammalian cells. Methods: PC12 cells were harvested from the rat end-stage PC12 cells. The ground control group, γ60Co-ray irradiation group, simulated microgravity effect control group, γ60 Co-ray irradiation simulation Micronucleus rate, micronucleus rate and frequency of HPRT gene mutation in microgravity effect group were analyzed to simulate the damage of mammalian cells in space environment. Results: The frequencies of micronuclei, micronucleus cells and HPRT gene mutations increased with the increase of dose of γ ~ (60) Co - radiation, which showed a good effect. However, γ ~ (60) Co - All the indicators in the simulated composite microgravity effect group were lower than that of γ ~ (60) Co-radiation group. Conclusion: γ ~ (60) Co - ray irradiation and simulated microgravity effects can both induce chromosome damage and HPRT gene mutation in PC12 cells, and the mechanism of mimicry microgravity effect on PC12 cell damage remains to be further studied the study.