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目的 :对汉族人胶质细胞源性神经营养因子 (GDNF)前体 c DNA进行核苷酸序列分析及功能研究。 方法 :通过RT- PCR法扩增汉族人 GDNF前体 c DNA ,利用昆虫杆状病毒表达系统 (BES)表达此前体 c DNA,原代培养中脑多巴胺能神经元对表达产物进行活性测定。结果 :汉族人 GDNF前体 c DNA为截短的 5 5 5 bp的转录体 ,其在昆虫细胞中的分泌表达产物能促进多巴胺能神经元的存活和分化。 结论 :汉族人 GDNF前体 c DNA序列与文献报道的 6 33 bp的序列相比缺失了 78个碱基 ,此 78个碱基的缺失并不影响人 GDNF蛋白的分泌及其活性
Objective: To investigate the nucleotide sequence and function of c DNA of GDNF precursor of Han nationality. Methods: The c DNA of pre-human GDNF precursor was amplified by RT-PCR. The c DNA was expressed by the insect baculovirus expression system (BES). The primary cultured human dopaminergic neurons were used to detect the expression of the expressed product. Results: The c DNA of GDNF precursor of Han nationality was a truncated 555 bp transcript. The secreted expression product of cDNF in insect cells could promote the survival and differentiation of dopaminergic neurons. CONCLUSION: The c DNA sequence of GDNF precursor in Han population is 78 bp shorter than the 633 bp reported in the literature. The deletion of 78 bp does not affect the secretion and activity of human GDNF protein