To study whether integrative fertilization [growing milk vetch in winter and reducing the dose of chemical nitrogen(N) fertilizer] can improve rice yield, and to reveal the underlying regulatory mechanisms for integrative fertilization, a three-year field trial including two treatments, milk vetch-rice-rice(MRR) and winter fallow-rice-rice(FRR), was conducted in 2010, 2011 and 2012.Our results demonstrated that the MRR treatment could significantly improve rice yield compared with the FRR treatment, especially when the application ratio of milk vetch and chemical fertilizer was 1:2.MRR treatment increased the effective panicle number and the spikelet number per panicle.In addition, a higher tillering number, leaf area index, photosynthetic-potential and photosynthetic-potential to grain ratio were observed in MRR treatment, which could provide enough dry matter for yield formation.Moreover, in MRR treatment, we discovered a higher transportation ratio and transformation ratio of dry matter in culm and leaves, and a stronger total sink capacity and spikelet-root bleeding intensity at the heading stage and 15 d after heading.Furthermore, the MRR treatment showed higher total N, phosphorus and potassium uptakes than FRR treatment, which was associated with the higher root dry weight in each soil layers.These results suggest that growing milk vetch in winter can improve rice yield under less chemical N fertilizer application, which is due to the improvement of soil nutrient status and the increased of rice root growth and development. To study whether integrative fertilization [growing milk vetch in winter and reducing the dose of chemical nitrogen (N) fertilizer] can improve rice yield, and to reveal the underlying regulatory mechanisms for integrative fertilization, a three-year field trial including two treatments, milk it results in the the MRR treatment could significantly improve rice yield compared with the FRR treatment, especially when the application of vetiver rice (MRR) and winter fallow-rice-rice (FRR), was conducted in 2010, 2011 and 2012. ratio of milk vetch and chemical fertilizer was 1: 2.MRR treatment increased the effective panicle number and the spikelet number per panicle. addition, a higher tillering number, leaf area index, photosynthetic-potential and photosynthetic-potential to grain ratio were observed in MRR treatment, which could provide enough dry matter for yield formation. Moreover, in MRR treatment, we discovered a higher transportation ratio and transformation ratio of dry ma tter in culm and leaves, and a stronger total sink capacity and spikelet-root bleeding intensity at the heading stage and 15 d after heading. Durthermore, the MRR treatment showed higher total N, phosphorus and potassium uptakes than FRR treatment, which was associated with the higher root dry weight in each soil layers. these results suggest that growing milk vetch in winter can improve rice yield under less chemical N fertilizer application, which is due to the improvement of soil nutrient status and the increased of rice root growth and development.