论文部分内容阅读
收集了8例正常人早期胎盘组织,采用PAP法显示人胎盘绒毛hCG的定位。观察结果表明仅合体滋养层细胞质内有免疫组织化学阳性反应,而合体滋养层细胞核、细胞滋养层细胞和绒毛轴心都是阴性反应。这结果证实了大多数学者的看法,即hCG定位在合体滋养层细胞质内。比较了三种免疫过氧化物酶方法的敏感性,肯定了PAP法优于酶标记抗体法,双PAP法又优于PAP法。由于PAP法不仅敏感性高而且特异性染色与非特异性染色之间的对比清楚,因此认为此法最可采用。 本工作还对不同固定液和孵育的不同温度和时间对PAP法染色的影响作了比较和讨论。
Eight normal early human placenta tissues were collected, and the localization of human placental hCG was detected by PAP method. Observations showed that only syncytiotrophoblast cytoplasm immunohistochemical positive reaction, and syncytiotrophoblast nucleus, trophoblast cells and villus axis are negative reactions. This result confirms the opinion of most scholars that hCG is localized in the syncytiotrophoblast cytoplasm. The sensitivity of three immunoperoxidase methods was compared, and the PAP method was superior to the enzyme labeled antibody method. The double-PAP method was superior to the PAP method. Due to the high sensitivity of the PAP method and the clear contrast between specific and nonspecific staining, it is considered that this method is the most suitable. In this work, we also compared and discussed the effect of different fixative and incubation temperature and time on PAP staining.