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目的:观察低浓度的毒毛旋花子苷原(Strophanthidin,Str)对离体豚鼠心脏是否有强心作用,及其与心肌细胞膜Na~+,K~+-ATP酶活性的关系。方法:采用Langendorff离体灌流装置经主动脉逆行灌流心脏;八道生理记录仪记录心率(HR),左室内压(LVP)及其最大变化速率(±dP/dt_(max));无机磷法测定心肌细胞膜Na~+,K~+-ATP酶活性。结果:Str 0.1nmol/L可兴奋Na~+,K~+-ATP酶活性(P<0.05)但不影响心脏的收缩功能,Str 1nmol/L仅能升高+dP/dt_(max)(P<0.05)并兴奋Na~+,K~+-ATP酶活性(P<0.01),Str 10和100 nmol/L可升高LVP和+dP/dt_(max)(P<0.05或P<0.01),对Na~+,K~+-ATP酶活性无明显作用,Str 1-100μmol/L虽能短暂升高LVP和±dp/dt_(max)(P<0.01),但随后出现不规则收缩并使LVP和±dP/dt_(max)降低,其对Na~+,K~+-ATP酶活性则表现为剂量依赖性抑制作用(P<0.01)。结论:高浓度Str的正性肌力作用是通过抑制Na~+,K~+-ATP酶活性实现的,而低浓度Str的强心作用似与Na~+,K~+-ATP酶抑制作用无关。
Objective: To observe whether low concentrations of Strophanthidin (Str) have cardiac effects on isolated guinea pig hearts and their relationship with Na ~ +, K ~ + -ATPase activity. Methods: The heart was retrograde perfusion with a Langendorff apparatus. Heart rate (HR), left ventricular pressure (LVP) and the maximum rate of change (± dP / dt max) were recorded by eight physiological loggers. Myocardial membrane Na ~ +, K ~ + -ATPase activity. Results: Str 0.1 nmol / L could activate Na +, K + -ATPase activity (P <0.05) but did not affect cardiac systolic function. Str + nmol / L increased only + dP / dt max (P <0.05 or P <0.01), and the activities of Na ~ +, K ~ + -ATPase were increased (P <0.01). StrP and 100 nmol / L increased LVP and + dP / dt max , There was no significant effect on Na ~ +, K ~ + -ATPase activity. Str 1-100μmol / L could temporarily increase LVP and ± dp / dtmax (P <0.01) LVP and ± dP / dt_ (max) decreased. The activities of Na ~ + and K ~ + -ATP showed a dose-dependent inhibition (P <0.01). Conclusion: The inotropic effect of Str at a high concentration is achieved by inhibiting the activity of Na ~ + and K ~ + -ATPase. However, the cardiac function of Str at a low concentration is similar to that of Na ~ + and K ~ + -ATPase Nothing to do