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本文以甘油、乙二醇、聚乙烯吡咯酮和二甲亚砜四种防护剂测试冻有16株单层细胞系和悬液细胞系,结果这四种保护剂都能适用于单层细胞系,甘油对悬液细胞的复苏不佳,冷冻保存期从1天至1562天不等,活力一般达80～100%,并可传代培养。冻存小管以0.2～0.5毫升的小量细胞悬液为宜,这样复苏时易速融,活力高,Me_2SO具有一定毒性,与细胞悬液混合时应在冰浴中进行,冻存和复苏时操作均要尽量快速。
In this paper, glycerol, ethylene glycol, polyvinylpyrrolidone and dimethyl sulfoxide four kinds of protective agent test freeze-16 monolayer cell lines and suspension cell lines, the results of these four protective agents can be applied to the monolayer cell line , Glycerol poor recovery of suspended cells, cryogenic preservation period from 1 day to 1562 days, the general activity of 80 to 100%, and can be subculture. Frozen small tube to 0.2 to 0.5 ml of a small amount of cell suspension is appropriate, so easy to melt fast recovery, high vitality, Me_2SO has some toxicity, mixed with the cell suspension should be carried out in an ice bath, cryopreservation and recovery The operation should be as fast as possible.