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目的:探讨参附注射液对大鼠心肺复苏后血清S100β蛋白、神经元特异性烯醇化酶和脑组织病理损伤的影响。方法:成年雄性SD大鼠160只,随机分为对照组、复苏组、小剂量参附组(10 mL/kg)和大剂量参附组(20 mL/kg),每组40只。建立窒息型大鼠心肺复苏模型,分别于各时间点(0.5 h、3 h、6 h、12 h和24 h)取血取样,ELISA法测定血清S100β蛋白、神经元特异性烯醇化酶(NSE)水平,光镜和电镜下观察大脑皮层组织病理改变。结果:复苏组和大、小剂量参附组血清S100β蛋白、NSE水平在ROSC后各个时间点均显著高于对照组(P<0.05或P<0.01);大、小剂量参附组与复苏组同时间比较,从ROSC后6h起血清S100β蛋白含量均显著降低(P<0.05或P<0.01);同复苏组相比,小剂量参附组ROSC后6h、12h血清NSE含量显著降低(P<0.05),大剂量参附组从ROSC后6h开始,血清NSE浓度显著降低(P<0.01);在ROSC后各个时相大、小剂量参附组间相比,血清S100β蛋白、NSE含量均无显著性差异(P>0.05)。光镜和电镜下组织病理改变均显示,大小剂量参附组大脑皮层组织结构损害程度明显轻于复苏组。结论:参附注射液尤其是大剂量应用对大鼠心肺复苏后脑缺血损伤具有较明显保护作用。
Objective: To investigate the effect of Shenfu injection on serum S100βprotein, neuron specific enolase (NSE) and brain tissue pathology in rats after cardiopulmonary resuscitation. Methods: 160 adult male Sprague-Dawley rats were randomly divided into control group, resuscitation group, small dose Shenfu group (10 mL / kg) and high dose Shenfu group (20 mL / kg), 40 rats in each group. The model of cardiopulmonary resuscitation in asphyxiated rat was established. The blood samples were collected at different time points (0.5 h, 3 h, 6 h, 12 h and 24 h). Serum levels of S100β protein, neuron specific enolase (NSE ) Levels, light microscopy and electron microscopy pathological changes in cerebral cortex tissue. Results: Serum levels of S100β protein and NSE in resuscitation group, large and small dose Shenfu group were significantly higher than those in control group at all time points (P <0.05 or P <0.01) Compared with the recovery group, the levels of S100β in serum of 6 h and 12 h after ROSC treatment were significantly decreased (P <0.05 or P <0.01) 0.05). Serum levels of NSE were significantly decreased at 6h after ROSC in high-dose Shenfuji group (P <0.01). Compared with the high-dose Shenfuji group, the levels of S100βprotein and NSE were not significantly different Significant difference (P> 0.05). Both light and electron microscopy histopathological changes showed that the size of the dose of reference group of cerebral cortex tissue damage was significantly lighter than the recovery group. Conclusion: Shenfu injection, especially high-dose application, has a significant protective effect on cerebral ischemic injury after cardiopulmonary resuscitation in rats.