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目的探究小鼠衣原体(Chlamydia)肺部感染中自然杀伤(NK)细胞对白细胞介素-22(IL-22)产生及疾病进程的影响。方法 36只小鼠分为实验组和对照组,每组18只。实验组小鼠尾静脉注射anti-asialo GM1,以封闭体内NK细胞,同时鼻吸入含鼠衣原体(Chlamydia muridarum)的感染液;对照组小鼠尾静脉注射同型对照抗体,感染方法同实验组。每天记录小鼠体质量变化;分别于感染后6、12 d,检测小鼠肺部衣原体包涵体形成单位(IFU);实时荧光定量PCR、ELISA方法检测小鼠脾脏中IL-22的表达,细胞内细胞因子染色技术检测小鼠脾脏Th17细胞数量变化。结果与对照组相比,实验组小鼠肺部感染加重,体质量降低趋势加大、肺部IFU增加(P<0.05),脾脏中IL-22的mRNA和蛋白表达水平减少(P<0.05),且Th17细胞的百分比和绝对数量均降低(P<0.01)。结论 NK细胞封闭导致小鼠体内IL-22的表达降低、肺部感染加重,提示NK细胞与IL-22的表达存在相关性;NK细胞可通过促进IL-22的产生,从而增强机体抗衣原体感染的能力。
Objective To investigate the effect of natural killer (NK) cells on interleukin-22 (IL-22) production and disease progression in mice with Chlamydia pneumonia. Methods 36 mice were divided into experimental group and control group, with 18 rats in each group. The mice in the experimental group were injected anti-asialo GM1 through the tail vein to block the NK cells in the body and nasal infection with Chlamydia muridarum. The mice in the control group were injected with the same type of control antibody through the tail vein, and the infection method was the same as the experimental group. The changes of body weight of mice were recorded daily. Inclusion body formation units (IFU) of lung were detected at 6 and 12 days after infection respectively. The expression of IL-22 in mouse spleen was detected by real-time fluorescence quantitative PCR and ELISA. Detection of Th17 cell number in mouse spleen by cytokine staining. Results Compared with the control group, the mice in the experimental group had increased pulmonary infection, decreased body weight, increased IFU in the lung (P <0.05), and decreased the mRNA and protein expression of IL-22 in the spleen (P <0.05) , And the percentage and absolute number of Th17 cells decreased (P <0.01). Conclusion NK cell blockade leads to the decrease of IL-22 expression and pulmonary infection in mice, suggesting that there is a correlation between NK cell and the expression of IL-22. NK cell can enhance the body’s anti-Chlamydial infection by promoting IL-22 production Ability.