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背景:为改善胶原支架的降解性能,作者对胶原支架的高温脱水物理交联方法进行了改进,将交联时间由24 h增加到48 h,将交联温度由105℃提高到115℃。目的:验证改进高温脱水物理交联方法制备胶原支架的抗降解能力,获取支架在体内对受损组织修复与再生的最佳功效。方法:将高纯度保持三螺旋结构的动物源性Ⅰ型胶原制成膜状支架,分别采取3种不同条件的高温脱水进行交联加强(105℃/24 h、105℃/48 h、115℃/24 h),将1 cm×1 cm的材料置入大白鼠背部皮下组织,在置入后第3,14,42天处死,取出标本进行组织学检查与分析。结果与结论:实验表明,3组内置物在机体内均未发现严重的异物及特异性免疫机能性反应,采用105℃/48 h加强条件处理的胶原支架,置入后14 d在体内的存留及保持孔隙开放程度均较其他两组好(P<0.05),间接表明将交联加强时间从常规24 h延至48 h可以增强胶原支架在体内的抗降解性能。
BACKGROUND: To improve the degradation performance of collagen scaffolds, the authors improved the physical crosslinking method of high temperature dehydration of collagen scaffolds. The crosslinking time increased from 24 h to 48 h and the crosslinking temperature increased from 105 ℃ to 115 ℃. OBJECTIVE: To verify the improved anti-degradation ability of collagen scaffolds prepared by high temperature dehydration physical crosslinking and to obtain the best effect of scaffolds for repairing and regenerating damaged tissues in vivo. METHODS: Animal-derived type Ⅰ collagen with a high degree of triple helix structure was made into a membrane-type scaffold. Three different conditions of high temperature dehydration were used to enhance the cross-linking (105 ℃ / 24 h, 105 ℃ / 48 h, 115 ℃ / 24 h), 1 cm × 1 cm material was implanted into the back of the rats subcutaneously. The rats were sacrificed on the 3rd, 14th, and 42th days after implantation. The specimens were removed for histological examination and analysis. RESULTS AND CONCLUSION: The experiment showed that none of the 3 groups of built-in objects found any serious foreign body and specific immune functional reaction in the body. Collagen stents treated with 105 ℃ / (P <0.05), which indirectly indicated that prolonging the crosslinking time from 24 h to 48 h could enhance the anti-degradation performance of collagen scaffolds in vivo.