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目的:观察雷公藤多苷对结肠炎小鼠外周血CD40/CD40L表达的影响。方法:小鼠随机分成正常组、模型组、雷公藤多苷灌肠、灌胃组及柳氮磺吡啶组,除正常组外,其它动物予TNBS/乙醇法复制溃疡性结肠炎动物模型,雷公藤多苷灌肠、灌胃组均给予20mg/kg,柳氮磺吡啶60mg/kg,正常组及模型对照组均予等容量生理盐水灌肠。通过观察小鼠体重、结肠湿重、结肠重量指数及病理变化和病理损伤评分评价雷公藤多苷治疗小鼠结肠炎的疗效,同时采用流式细胞术检测其外周血CD40/CD40L表达,酶联免疫法检测结肠组织匀浆IL-1β和IL-4水平。结果:与模型组比较,20mg/kg雷公藤多苷灌肠、灌胃组小鼠结肠湿重和结肠重量指数结肠病理损伤评分明显低于模型组,且均有显著性统计学意义(P<0.05或P<0.01)。而且治疗组形态学可见黏膜上皮完整或少量受损,炎细胞散在性分布,基底层可见少量纤维结缔组织增生,溃疡修复。灌肠、灌胃及柳氮磺吡啶组则明显降低外周血CD40,升高CD40L表达(P<0.01),同时降低结肠组织匀浆中IL-1β表达、和升高IL-4表达水平(P<0.05或P<0.01),但灌胃、灌肠组间比较未见明显差异。结论:雷公藤多苷可能通过调节CD40/CD40L表达水平,抑制促炎因子表达,升高抗炎因子水平而有效缓解溃疡性结肠炎结肠粘膜损伤。
Objective: To observe the effects of Tripterygium on the expression of CD40 / CD40L in colitis mice. Methods: The mice were randomly divided into normal group, model group, tripterygium glycosides enema, gavage group and sulfasalazine group. All the animals except the normal group were given TNBS / ethanol to establish an animal model of ulcerative colitis. Tripterygium wilfordii Polyglycosidic enema, gavage group were given 20mg / kg, sulfasalazine 60mg / kg, normal group and model control group were given the same volume of saline enema. The therapeutic effect of tripterygium glycosides on colitis in mice was evaluated by observing the body weight, wet weight of colon, colon weight index, histopathological changes and pathological damage scores. Meanwhile, the expression of CD40 / CD40L in peripheral blood was detected by flow cytometry. Immunoassay was used to detect IL-1β and IL-4 in colonic tissue homogenate. Results: Compared with the model group, the scores of colon wet weight and colon weight index of pathological damage in colon and gut of 20 mg / kg tripterygium glycosides were significantly lower than those in model group (P <0.05) Or P <0.01). And morphological changes in the treatment group showed complete or a small amount of mucosal epithelial damage, inflammatory cells scattered distribution, the basal layer shows a small amount of fibrous connective tissue hyperplasia, ulcer repair. Enema, gavage and sulfasalazine significantly decreased peripheral blood CD40, increased CD40L expression (P <0.01), decreased IL-1β expression in colonic tissue and increased IL-4 expression (P < 0.05 or P <0.01), but there was no significant difference between gavage and enema groups. CONCLUSION: Tripterygium glycosides may effectively relieve colonic mucosal damage of ulcerative colitis by regulating the expression of CD40 / CD40L, inhibiting the expression of proinflammatory cytokines and elevating the level of anti-inflammatory cytokines.