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神经母细胞瘤和神经胶质瘤细胞融合的克隆细胞系ＮＧ１０８－１５细胞在含分化剂双丁基环化单磷酸腺苷（ｄＢｆｃＡＭＰ）的培养液培养后分化，成为具神经细胞特征的细胞。本实验利用四唑盐（ＭＴＴ）微量比色法，并结合焦油紫染色，测定和观察细胞的生长及分化状况，研究了低氧（２％Ｏ２＋９３％Ｎ２＋５／ＣＯ２）对未分化的，分化中的和已分化完成的ＮＧ１０８－１５细胞的影响。获得的主要结果是；低氧明显降低未分化细胞增殖和存活率，使分化完成的细胞大量死亡；低氧影响ＮＧ１０８－１５细胞的分化，使细胞在分化中出现体积膨大，突起短等异常特征，经焦油紫染色，胞质中无尼氏体（即不着色）的细胞增多。低氧是否可能使未分化ＮＧ１０８－１５细胞向更多地表达胶质细胞特征的方向分化？将是一个十分有趣的问题。
The cloned cell line NG108-15 cells fused with neuroblastoma and glioma cells differentiated into neuronal cells after they were cultured in the culture medium containing the bisdyl cyclized monophosphate adenine dinucleotide (dBfcAMP). In this study, MTT micro-colorimetry was used in combination with tar violet staining to measure and observe the cell growth and differentiation. The effects of hypoxia (2% O2 + 93% N2 + 5 / CO2) on the differentiation, And the differentiation has been completed by the impact of NG108-15 cells. The main results obtained are: hypoxia significantly reduced the proliferation and survival rate of undifferentiated cells, resulting in a large number of differentiated cells died; hypoxia affect the differentiation of NG108-15 cells so that the cells appear in the differentiation of volume expansion, short and other abnormal features , Staining with tar violet and increasing in the cytoplasm of non-Ni (ie non-colored) cells. Is hypoxia possible to differentiate undifferentiated NG108-15 cells into more glial cells? It will be a very interesting question.