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目的:观察人工虫草菌丝对CCl_4中毒性肝纤维化的预防作用。方法:复制大鼠CCl_4中毒性肝纤维化模型,并以秋水仙碱作为阳性治疗对照,采用光镜、电镜观察肝脏组织学改变,测定血清ALT、Alb、G、HA、LN、肝组织Hyp含量。结果:虫草菌丝组肝细胞损害、肝脏脂肪变性、炎性细胞浸润的程度均较模型组轻,但再生肝细胞较多,有假小叶形成;其对肝功能、肝纤维化指标也有明显改善,但秋水仙碱的抗肝纤维化作用优于虫草菌丝。结论:虫草菌丝对肝脏CCl_4损伤具有一定保护作用,有较强促进肝细胞再生修复作用,同时在损伤肝组织中成纤维细胞及胶原纤维也明显增生,其与活血化瘀药有机伍用,可能是防治肝纤维化较理想的组合。
Objective: To observe the preventive effect of Cordyceps mycelium on CCl_4 toxic hepatic fibrosis. METHODS: The model of hepatic fibrosis in rat CCl_4 was duplicated. Colchicine was used as a positive control. The liver histological changes were observed by light and electron microscopy. Serum ALT, Alb, G, HA, LN, liver tissue Hyp were determined. . RESULTS: Cordyceps mycelium had less hepatocyte damage, hepatic steatosis, and inflammatory cell infiltration than the model group. However, there were more regenerative hepatocytes and false lobule formation. The liver function and hepatic fibrosis indexes also improved significantly. , But colchicine’s anti-fibrotic effect is better than Cordyceps mycelium. Conclusion: Cordyceps mycelium has a protective effect on liver CCl_4 injury, and it has a stronger effect on promoting the regeneration and repair of hepatocytes. At the same time, the fibroblasts and collagen fibers in the damaged hepatic tissue also proliferate obviously. They are used organically with blood circulation and stasis drugs. It may be an ideal combination to prevent and treat liver fibrosis.