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目的探讨605nm羧基水溶性量子点在鼻咽癌标志物EB病毒核抗原1(EBNA1)中的标记方法。方法将605nm羧基水溶性量子点和鼻咽癌EBNA1在1-(3-二甲氨基丙基)-3-乙基碳二亚胺盐酸盐的作用下共价交联,生成量子点标记的抗原,对标记后的抗原采用紫外可见吸收谱、荧光光谱测定,琼脂糖凝胶电泳分析。结果羧基水溶性量子点与鼻咽癌EBNA1抗原通过表面氨基与羧基缩合形成的稳定共价键,羧基水溶性量子点与鼻咽癌EBNA1抗原之间实现了成功连接,标记后荧光发射光谱检测结果表明,标记后的量子点EBNA1溶液的发射峰位置在380nm附近,保持良好的荧光特性。结论 605nm羧基水溶性量子点采用共价交联法可稳定标记鼻咽癌标志物EBNA1,为后续研究提供可靠依据。
OBJECTIVE To investigate the labeling of 605nm carboxy-water soluble quantum dots in nasopharyngeal carcinoma marker Epstein-Barr virus nuclear antigen 1 (EBNA1). Methods The quantum dots of 605nm carboxyl group and nasopharyngeal carcinoma EBNA1 were covalently cross-linked by 1- (3-dimethylaminopropyl) -3-ethylcarbodiimide hydrochloride to generate quantum dot-labeled Antigen, the labeled antigen using UV-visible absorption spectrum, fluorescence spectrometry, agarose gel electrophoresis analysis. Results The carboxyl group of water-soluble QDs and nasopharyngeal carcinoma EBNA1 antigen formed a stable covalent bond through the condensation of amino groups and carboxyl groups on the surface. The successful connection of the carboxyl group of water-soluble quantum dots and EBNA1 antigen of nasopharyngeal carcinoma was achieved. The labeled fluorescence emission spectrum The results show that the emission peak position of labeled EBNA1 quantum dots is around 380nm, which keeps good fluorescence characteristics. CONCLUSION: The covalent cross-linking method can stably label EBNA1, a marker of nasopharyngeal carcinoma, in 605nm carboxyl water-soluble quantum dots, which can provide a reliable basis for subsequent research.