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为研究我国云南省彝族、傣族人群ｖＷＦ基因ＨｈａⅠ和ＳｍａⅠ限制性片段长度多态性特点，应用多聚酶链式反应技术结合ＨｈａⅠ和ＳｍａⅠ酶切分析研究了４４个彝族人７８条染色体、３９个傣族人８８条染色体等位基因的杂合情况。结果在彝族人ＨｈａⅠ＋ＨｈａⅠ－和ＳｍａⅠ＋ＳｍａⅠ－为０．３６０．６４；在傣族人中ＨｈａⅠ＋ＨｈａⅠ－和ＳｍａⅠ＋ＳｍａⅠ－为０．４３０．５７，且发现ＨｈａⅠ和ＳｍａⅠ在两民族中呈完全连锁，未发现ＨｈａⅠ＋／ＳｍａⅠ－或ＨｈａⅠ＋／ＳｍａⅠ－染色体。提示在我国彝、傣两少数民族中，在ｖＷＦ基因的５端均存在ＨｈａⅠ和ＳｍａⅠ的多态性位点，且具有较高的理论杂合率，可用于ｖＷＤ的遗传咨询和产前诊断。
In order to study the characteristics of the restriction fragment length polymorphism (HHA) and SmaI (restriction fragment length polymorphism) of vWF gene in Yi and Dai populations of Yunnan province in China, polymerase chain reaction (PCR) and HhaⅠ and SmaⅠ restriction analysis were used to study the genetic diversity of 44 Yi populations, 78 chromosomes, 39 Dai individuals 88 chromosome alleles heterozygous situation. The results showed that HhaⅠ + HhaⅠ- and SmaⅠ + SmaⅠ- were 0.360.64 in Yi people; HhaⅠ + HhaⅠ- and SmaⅠ + SmaⅠ- were 0.430.57 in Dai people, and HhaⅠ and SmaⅠ were found to be completely linked in the two ethnic groups. No HhaⅠ + / SmaⅠ - or HhaI + / SmaI- chromosomes. It is suggested that polymorphisms of Hha I and Sma I exist at the 5 end of vWF gene in Yi and Dai ethnic minorities in our country and have high theoretical heterozygosity and may be used for genetic counseling and prenatal diagnosis of vWD .