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儿童急性淋巴细胞性白血病(Acute Lymphoblastic Leukemia,ALL)是儿童常见的恶性肿瘤.尽管已有研究报道miRNA在ALL发病机理中起重要调控作用,但其与ALL复发的关系目前还不完全清楚.本研究采用第二代高通量测序技术分别对一例B淋巴细胞ALL(B-ALL)和一例T淋巴细胞ALL(T-ALL)患儿初发和复发期的骨髓细胞进行miRNA检测,比较初发和复发期样本miRNA的表达差异.在T-ALL和B-ALL中分别鉴定出复发期较初发期表达发生明显变化的miRNA 168和123种.其中在T-ALL样本中,复发期表达上调的miRNA有115种,表达下调的有53种;而在B-ALL样本中,复发期表达上调的miRNA只有38种,表达下调的有85种.比较两种类型ALL,共有17种miRNA在两种类型中发生相同的改变.通过realtime RT-PCR验证,证实Hsa-miR-17在复发期ALL中明显升高,而Hsa-miR-10a则明显降低.
Acute Lymphoblastic Leukemia (ALL) is a common malignant tumor in children.Although miRNAs have been reported to play an important regulatory role in the pathogenesis of ALL, the relationship between them and the relapse of ALL is not fully understood The second generation of high-throughput sequencing technique was used to detect the miRNA of bone marrow cells in primary and recurrent patients of one case of B lymphocyte ALL (B-ALL) and one case of T lymphocyte ALL (T-ALL) And recurrent miRNA expression.Among them, 168 and 123 miRNAs were detected in T-ALL and B-ALL, respectively, which were significantly higher than those in the initial stage of recurrence.Among these T-ALL samples, the expression of miRNA was up-regulated Of the 115 miRNAs were downregulated in 53 species, whereas in B-ALL samples, only 38 miRNAs were upregulated at recurrence and 85 were down-regulated Hsa-miR-10a was significantly increased in ALL and Hsa-miR-10a was significantly reduced by realtime RT-PCR.