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目的对比分析妊娠晚期腹腔注射缓冲液对近交系SPF级C57BL/6J(B6)和BALB/c(B/c)小鼠繁育生理的影响。方法 B6和B/c小鼠随机、全同胞兄妹2∶1/1∶1(♀/♂)过夜同居交配,观察交配方式、阴栓与受孕率的关系;受孕鼠在妊娠的第17.5天(妊娠0d=交配后当天)接受腹腔注射酸性磷酸缓冲液,观察注射对母鼠及胚胎的作用及子鼠从离乳到8周的早期生长发育情况。结果 B6较B/c雌鼠的受孕率高(29.4%vs.21.1%[2∶1],33.2%vs.29.7%[1∶1]);交配后10 d～14 d,根据雌鼠增大的腹部、结合体重来判断受孕较观察阴栓更为准确;比较而言,妊娠晚期腹腔注射对B6母鼠及胚胎的影响较大,表现在离乳子鼠数量减少(4.7±3.1 vs.6.1±2.1,P=0.231),离乳时两性别的子鼠体重(g,雌性:11.7±1.1 vs.12.7±1.5;雄性:12.8±1.3 vs.13.6±1.5)显著降低(P均<0.05)及两品系子鼠早期生长发育的方式显著不同(P=0.000)。结论近交系小鼠繁殖生理存在品系差异;两品系受孕鼠对妊娠晚期腹腔注射的耐受不同,并可能影响实验动物产后的哺乳过程及子鼠的早期生长发育。
Objective To compare and analyze the effects of intraperitoneal injection of third trimester of pregnancy on the reproductive physiology of inbred strains of SPF C57BL / 6J (B6) and BALB / c (B / c) mice. Methods B6 and B / c mice were randomly assigned to sibling siblings 2: 1/1 (♀ / ♂) for cohabitation overnight to observe the relationship between copulation methods and vaginal suppository and pregnancy rate. On the 17th day of gestation Pregnancy 0d = the day after mating) received intraperitoneal injections of acid phosphate buffer to observe the effects of injection on maternal mice and embryos and the early growth and development of offspring from offspring to 8 weeks. Results The pregnancy rate of B6 was significantly higher than that of B / C female mice (29.4% vs.21.1% [2:1], 33.2% vs.29.7% [1: 1]). Large belly, combined with body weight to determine the pregnancy than the observation of the vaginal suppository more accurate; comparatively, late pregnancy, intraperitoneal injection of B6 greater impact on embryos and embryos, showing a decrease in the number of offspring mice (4.7 ± 3.1 vs. 6.1 ± 2.1, P = 0.231). Body weight (g, females: 11.7 ± 1.1 vs.12.7 ± 1.5; males: 12.8 ± 1.3 vs.13.6 ± 1.5) ) And two-way offspring early growth and development of the way significantly different (P = 0.000). Conclusions The inbred strains of inbred mice have differences in reproductive physiology. The inbred mice of the two strains have different tolerance to the intraperitoneal injection in the third trimester, and may affect the postpartum lactation process and the early growth of the offspring.