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背景和目的 人类FUT2基因决定ABH分泌状态并影响个体Lewis表型。本项研究基于台湾本土Lewis(a+b-)非分泌不同人群,确定其se基因型。方法 血液样品中Lewis表型确定乃通过微量板方法。Lewis(a+b-)表型个体其se基因型分析乃采用PCR-限制性片断长度多态性(PCR-RFLP)方法,其根据se等位基因设计以前已有报导。PCR及克隆技术用于新se基因编码序列的确定。结果 一新的se等位基因,se685,被确认为在编码位点核苷685～689序列中,有3核苷缺失,导致GTGGT→GT,这是在1例台湾本土Ami族Le(a+b-)非分泌
Background and Objective The human FUT2 gene determines ABH secretion status and affects individuals’ Lewis phenotypes. This study based on Taiwan’s native Lewis (a + b-) non-secreting different populations to determine their se genotype. Methods Lewis phenotype determination in blood samples was performed by a microplate method. The se genotype analysis of individuals with the Lewis (a + b-) phenotype using PCR-restriction fragment length polymorphism (PCR-RFLP) has been previously reported based on the se allele design. PCR and cloning techniques for the determination of the new se gene coding sequence. Results A new se allele, se685, was found to have a 3-nucleoside deletion at nucleotide 685-689 in the coding sequence, resulting in GTGGT → GT, which was found in a case of Taiwan native Ami Le (a + b-) Non-secreted